Table 1. Zinc binding and stability of LECT2.

Conditions were 2.5 μM protein, 20 mM Tris (pH 7.5), 0.15 M NaCl, 37 °C. Holo LECT2 samples contained 2.5 μM ZnCl₂ and 7.5 μM iminodiacetic acid. Errors are s.d. (n = 3 – 4). NA, not applicable.

Protein	Kd (nM)	koff (s−1)	ΔGfold (kcal/mol)
WT LECT2	0.14 ± 0.03	0.0032 ± 0.0002	−6.31 ± 0.49
I40V LECT2	0.21 ± 0.03	0.0025 ± 0.0002	−6.69 ± 0.12
WT apoLECT2	NA	NA	−3.35 ± 0.52
I40V apoLECT2	NA	NA	−3.04 ± 0.08
WT LECT2-taga	0.79 ± 0.45b	0.0098 ± 0.0021	−4.16 ± 0.10
I40V LECT2-taga	0.76 ± 0.27b	0.0093 ± 0.0010	−3.90 ± 0.006
WT apoLECT2-taga	NA	NA	−2.65 ± 0.05
I40V apoLECT2-taga	NA	NA	−2.18 ± 0.02

^aHa et al. (2021)⁷.

^b22 °C.