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[Preprint]. 2023 Jul 15:2023.07.13.548265. [Version 1] doi: 10.1101/2023.07.13.548265

Table 1.

Candidate default ascending arousal network (dAAN) nodes.

Candidate dAAN node Supporting Literature
Study author, year Animal model Type of Evidence
Brainstem
Midbrain reticular formation (mRt) Steriade, 1982 (35) cat electrophysiology
Ventral tegmental area (VTA) Eban-Rothschild, 2016 (19) mouse electrophysiology
Periacqueductal grey (PAG) Lu, 2006 (36) rat gene expression
Pedunculotegmental nucleus (PTg) Steriade, 1990 (33) cat electrophysiology
Dorsal raphe (DR) McGinty, 1976 (37) Trulson, 1979 (27) cat electrophysiology
Pontis oralis (PnO) Xi, 2004 (38) cat Agonist/antagonist injection
Parabrachial complex (PBC) Fuller, 2011 (6) Qiu 2016 (39) rat Lesion (Fuller) Stimulation (Qiu)
Median raphe (MnR) Trulson, 1984 (40) cat electrophysiology
Locus coeruleus (LC) Hobson, 1975 (26) Aston-Jones, 1981 (41) rat (Aston-Jones), cat (Hobson) electrophysiology
Laterodorsal tegmental nucleus (LDTg) Steriade, 1990 (33) cat electrophysiology
Hypothalamus
Tuberomammillary nucleus (TMN) Takahashi, 2006 (42) mouse electrophysiology
Lateral hypothalamic area (LHA) Alam, 2002 (43) rat electrophysiology
Supramammillary nucleus (SUM) Pedersen, 2017 (7) mouse chemogenetics
Thalamus
Intralaminar nuclei (IL) Glenn, 1982 (22) cat electrophysiology
Reticular nucleus (Ret) Steriade, 1986 (44) cat electrophysiology
Paraventricular nucleus (PaV) Novak, 1998 (45) rat gene expression
Basal Forebrain
Nucleus basalis of Meynert/substantia innominata (BNM/SI) Szymusiak, 1986 (46) cat electrophysiology
Diagonal band of Broca (DBB) Szymusiak, 1986 (46) cat electrophysiology

We identified 18 nodes that met the a priori criteria for inclusion in the human dAAN. The criteria were based on previous animal studies demonstrating nodal involvement in arousal from electrophysiological recording, Fos expression, lesioning, or stimulation. Given that decades of experiments in multiple animal species have tested the functional properties of subcortical arousal nuclei, we do not intend for the references in this table to be exhaustive. Rather, we list studies that are representative of the body of literature pertaining to each subcortical arousal nucleus.