Fig. 4. Nb-Ft-TRPV1^Ca2+ increases c-fos expression and cerebral metabolism upon DMF treatment.

A. Immunostaining for c-fos (cyan) and HA or RFP (red) of dorsal striatum of A2a-Cre mice euthanized 1 hour following 15 minutes of DMF exposure. B. Percentage (%) of c-fos positive (+) neurons over total number of iSPNs expressing HA or RFP (n=5 mice per group/3 sections per mice). C. Correlation between c-fos expression and freezing of gait during DMF exposure (n=5 mice per group). D. Protocol and schema for 18-FDG micro-PET/CT scans procedure and analysis with representative micro-PET/CT imaging of A2a-Cre mouse brains expressing the Nb-Ft-TRPV1^Ca2+ or the mCherry control virus following DMF exposure. 18-FDG uptake in dorsal striatum calculated as the percentage of injected dose/weight (%ID/g), E. DMF over baseline ratio. F. DMF over baseline normalized to cerebellum, in mCherry (blue bars/dots, n=8) and Nb-Ft-TRPV1^Ca2+ (red bars/dots, n=8) mice. G. Correlation between DMF over baseline 18-FDG uptake ratio and freezing of gait during DMF exposure (n=10). Error bars show SEM. * represents p value < 0.05, ** represents p value < 0.01, *** represents p value < 0.001 with two-tailed, unpaired t-test with Welch’s correction. Anti-HA staining to detect Nb-Ft-TRVP1^Ca2+ expression, anti-RFP staining to detect mCherry expression.