Fig. 5. Nb-Ft-TRPV1^Ca2+ increases calcium transients in dorsal striatum.

A. Schema of the double-floxed Cre-dependent AAV vector expressing the Nb-Ft-TRPV1^Ca2+ or mCherry under the control of the JET promoter (red) combined with double floxed Cre-dependent AAV vector expressing GCaMP6s under the control of the Syn promoter (green). Behavioral methodology to assess calcium transients during pre-DMF (green), DMF (red), and post-DMF (blue) treatment. B. Activity trace example of altered motor activity during bilateral striatal pre-DMF (green), DMF (red), and post-DMF (blue) stimulation in an Nb-Ft-TRPV1^Ca2+ mouse with heatmap of GCaMP fluorescence aligned to pre-DMF (left), DMF (middle), and post-DMF (right) of the same animal shown in activity trace example expressing the Nb-Ft-TRPV1^Ca2+ vector. C. Mean fluorescence aligned to pre-DMF, DMF, and post-DMF in Nb-Ft-TRPV1^Ca2+ group (n=3). D. Activity trace example of altered motor activity during bilateral striatal pre-DMF (green), DMF (red), and post-DMF (blue) stimulation in an mCherry mouse with heatmap of GCaMP fluorescence aligned to pre-DMF (left), DMF (middle), and post-DMF (right) for the same animal shown in activity trace example expressing the mCherry vector. E. Mean fluorescence aligned to pre-DMF, DMF, and post-DMF in the mCherry group (n=4) F. Freezing of gait (FOG), G. Average delta (Δ) F/F (%) during pre-DMF, DMF, and post-DMF stimulation. H. Correlation between ΔF/F (%) calcium transients and FOG during DMF treatment. Error bars show SEM. * represents p value < 0.05 with two-tailed, unpaired t-test with Welch’s correction.