Fig. 7. Selective viral-mediated expression of Nb-Ft-TRPV1^Ca2+ in the striatopallidal pathway elicit parkinsonian motor behavior in wild type mice.

A. Schema of the double-floxed Cre-dependent AAV vector expressing the Nb-Ft-TRPV1^Ca2+ or mCherry under the control of the JET promoter in striatal iSPN projecting to the Gp achieved by Gp injection of a retrograde AAV2 vector carrying Cre-GFP, and immunostaining for GFP (green), HA (red) in wt mice demonstrates selective expression of the AAV- Nb-Ft-TRPV1^Ca2+ in dorsal striatum iSPN projecting to the Gpe, white arrows show colocalization of GFP with HA. B. Example of altered motor activity during bilateral striatopallidal pre-DMF (green), DMF (red) and post-DMF (blue) stimulation, lines represent the mouse’s path. Effect of DMF stimulation on C. Ambulation bout duration, and D. Freezing of gait in mCherry (blue bars/dots, n=8) and Nb-Ft-TRPV1^Ca2+ (red bars/dots, n=8) mice. E. Immunostaining for c-fos (cyan) and HA (Nb-Ft-TRVP1^Ca2+; red) or RFP (mCherry; red) of dorsal striatum of wt mice euthanized 1 hour following 15 minutes of DMF exposure. White arrows show co-localization. F. Percentage (%) of c-fos positive (+) neurons over total number of iSPNs expressing HA or RFP (n=3 mice per group/3 sections per mice). G. Schema of the double-floxed Cre-dependent AAV vector expressing the Nb-Ft-TRPV1^Ca2+ or TRPV1^Ca2+ under the control of the JET promoter in striatal iSPNs projecting to the Gp achieved by Gp injection of a retrograde AAV2 vector carrying Cre-GFP. Effect of DMF stimulation on H. Ambulation bout duration, and I. Freezing of gait in TRPV1^Ca2+ (blue bars/dots, n=5) and Nb-Ft-TRPV1^Ca2+ (red bars/dots, n=7) mice. Error bars show SEM. ** represents p value < 0.01, *** represents p value < 0.001 with two-tailed, unpaired t-test with Welch’s correction .