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[Preprint]. 2024 Apr 29:2023.07.13.548699. Originally published 2023 Jul 15. [Version 2] doi: 10.1101/2023.07.13.548699

Fig. 2. Selective viral-mediated expression of Nb-Ft-TRVP1Ca2+ in striatal iSPNs elicit parkinsonian motor behavior.

Fig. 2.

A. Schema of the double-floxed Cre-dependent AAV vector expressing the Nb-Ft-TRVP1Ca2+ or mCherry under the control of the JET promoter, and immunostaining for HA in Adora-2A (A2a)-Cre mice demonstrates selective expression of the AAV- Nb-Ft-TRVP1Ca2+ in dorsal striatum. B. Selective transduction of D2-type iSPN with RNA in-situ hybridization (ISH). Co-localization of D2-type (green) iSPN with mCherry (red). C. Protocol and Schema for direct magnetic field (DMF) neural activation to assess motor behavior. D. Example of altered motor activity during bilateral striatal pre-DMF (green), DMF (red) and post-DMF (blue) stimulation; individual lines represent the path of a single mouse. Effect of DMF stimulation on E. Ambulation bout duration, and F. Freezing of gait in mCherry (blue bars/dots, n=10) and Nb-Ft-TRVP1Ca2+ (red bars/dots, n=10) mice. Error bars show SEM. ** represents p value < 0.01, **** represents p value <0.0001 with two-tailed, unpaired t-test with Welch’s correction .