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[Preprint]. 2023 Oct 11:2023.07.13.548906. Originally published 2023 Jul 13. [Version 2] doi: 10.1101/2023.07.13.548906

Figure 3: Disruption of the super-enhancer region by loss of CTCF binding impacts multiple genes.

Figure 3:

A. Location of CTCF binding site (CBS) at the 5’ and 3’ end of the super enhancer. Red box: Super-enhancer; Orange box and line: CTCF binding area. Orange arrow: Orientation of CFCT-binding sites. B. Diagram of convergent CTCF-binding at the base of loops. CTCF-binding sites 1 or 2 are potentially bind to 3 or 5 with high efficiency due to convergent orientation. The divergent orientation potentially formed between 1 or 2 and 4 is dis-favoured. Hence, 4 was excluded in the carton and not studied here for double mutation of CTCF binding site. C. CHIP-qPCR analysis for CTCF binding in wild type and CBS-mut cells. DD. qRT-PCR analysis of gene expression in single CBS-mut cells. Scramble gRNA infected cells were used as a control. Data are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005. n = 3. E. qRT-PCR analysis of gene expression in double CBS-mut cells. Data are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005. n = 3.