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[Preprint]. 2023 Oct 11:2023.07.13.548906. Originally published 2023 Jul 13. [Version 2] doi: 10.1101/2023.07.13.548906

Figure 4. Role of R1 enhancer region in β-cell function.

Figure 4.

A. Diagram of R1 enhancer deletion in EndoC-βH3 by CRISPR-Cas9 mediated genome editing. Two gRNAs were designed to delete either entire R1 region (R1-del1 by gRNA1 and gRNA2) or a smaller region containing two variants (R1-del2 by gRNA3 and gRNA2). In both cases, deletions resulted into the loss of exon 8 of the SLC30A8 gene, thus may affect ZnT8 translation. B. Taqman™ qRT-PCR analysis of gene expression in R1-del cells. C. Glucose stimulated insulin secretion (GSIS) assay. Data are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005. D. Fold change of secreted insulin. Data were normalized to insulin secretion at basal level (0.5 mM glucose). n = 3.