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[Preprint]. 2023 Oct 11:2023.07.13.548906. Originally published 2023 Jul 13. [Version 2] doi: 10.1101/2023.07.13.548906

Figure 5. Role of R2 region in β-cell function.

Figure 5.

A. Diagram of R2 deletion in EndoC-βH3 by CRISPR-Cas9 mediated genome editing. Two gRNAs were designed to delete entire R2 region (R2-del1 by gRNA1 and gRNA2) or a region containing the variants (R2-del2 by gRNA3 and gRNA2). B. Taqman™ qRT-PCR analysis of gene expression in R2-del cells. C. Glucose stimulated insulin secretion (GSIS) assay. Data are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005. Fold change of secreted insulin. Data were normalized to insulin secretion at basal level (0.5 mM glucose). n = 3.