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[Preprint]. 2023 Oct 11:2023.07.13.548906. Originally published 2023 Jul 13. [Version 2] doi: 10.1101/2023.07.13.548906

Figure 8. JQ1 increases insulin production and secretion in insulin producing cells.

Figure 8

A. Total INSULIN content in JQ1 treated EndoC-βH3 cells. 7 × 104 EndoC-βH3 cells were seeded in 96-well plate and incubated with different concentrations of JQ1 for 4 days. B. Fold change. DMSO treated sample was used as a control. C. Taqman™ RT-qPCR analysis of SLC30A8 and nearby genes in JQ1-treated EndoC-βH3 cells. EndoC-βH3 cells were treated with Tamoxifen (5 μm) for 3 weeks to remove gene cassette and increase insulin production [59]. Then, 1 × 106 cells were seeded in 6-well plate and incubated with JQ1 for 4 days. D and E. Glucose induced insulin secretion (GSIS) assay in EndoC-βH3 cells. 7 × 104 Tamoxifen treated cells were seeded in 96-well plate and incubated with JQ1 for 4 days. Cells were than preincubated with 2.8 mM glucose culture medium overnight before GSIS assay was performed. All Data above are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.005. n = 3.