Figure 4:

Initial biological evaluation with cell proliferation assays and photophysical properties of OptoLat. a) Antiproliferative activity of photoswitchable latrunculin analogs and reference compounds. b) Cell viability dose-response curves for 9 under dark and illuminated conditions. c) Cell viability dose-response curves for OptoLat (14) under illumination with different wavelengths and in the dark. d) Photochemical and thermal isomerization of OptoLat. e) UV-Vis absorption spectrum of OptoLat. f) Percentage of cis OptoLat measured by NMR with in situ irradiation at different wavelegths. g) Thermal relaxation of of cis OptoLat in the dark in DMSO solution at 37 °C. h) switching of OptoLat is reversible and fatigue-resistant over multiple cycles.