Table 1.
Cell isolation strategy
| Cell type | Surface marker | Ref. | Biological replicates (Normal) | Biological replicates (PHx 24hr) |
|---|---|---|---|---|
| biliary epithelial cholangiocytes (BEC) | ST14−CD26−MIC1–1C3+CD31CD45−CD11b− | Li et al, Stem Cell Reports, 2017 | 4 | 2 |
| Blood | CD45+ | Alaverdi, Current protocols in immunology, 2004 | 2 | 2 |
| Clonal biliary epithelial cholangiocytes (cBEC) | ST14+CD26−MIC1–1C3+CD31CD45−CD11b− | Li et al, Stem Cell Reports, 2017 | 4 | 2 |
| Endothelium (EC) | Lyve1−CD34+CD144+CD309+CD45− | Ding et al, Nature, 2010 | 2 | 3 |
| Hepatocyte (HC) | OC2–2F8+CD45−CD31− | Huch et al, Nature, 2013 | 4 | 4 |
| Hepatic Stellate (HSC) | CD146+CD45−CD31−Violate+ | Mederacke et al, Nature Protocols, 2015 | 2 | 3 |
| Kupffer (KC) | F4/80+CD11b+ | Kumar et al, JCI, 2006 | 2 | 3 |
| Liver Sinusoidal endothelium(LSEC) | Lyve1+CD34−CD144+CD309+CD45− | Ding et al, Nature, 2010 | 3 | 5 |
| Non-progenitor ducts(NPD) | CD26+MIC1–1C3+CD31−CD45CD11b− | Dorrell et al, Gene & Dev 2011 | 2 | 2 |
| Thy1 | Thy1+CD45− | Hoppo et al, Hepatology, 2004 | 2 | 2 |