Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix | Corning | Cat.# 354230 |
| Essential 8™ (E8) medium | Thermo Fisher Scientific | Cat.# A1517001 |
| Penicillin-Streptomycin | Lonza | Cat.# 17-602F |
| 10μM ROCK inhibitor Y-27632 | Abcam | Cat.# ab120129 |
| Chroman 1 | Torcis | Cat.# 7163 |
| Emricasan | MedKoo Biosciences | Cat.# 510230 |
| Polyamines | Sigma-Aldrich | Cat.# P8483 |
| Trans-ISRIB | Torcis | Cat.# 5284 |
| CHIR99021 | STEMCELL Technologies | Cat.# 72052 |
| IWP4 | STEMCELL Technologies | Cat.# 72552 |
| RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES | Thermo Fisher Scientific | Cat.# 72400047 |
| B-27 Supplement, minus insulin | Thermo Fisher Scientific | Cat.# A1895601 |
| AccuMax™ | STEMCELL Technologies | Cat.# 07921 |
| Bovine Serum Albumin (BSA) solution | Sigma-Aldrich | Cat.# A8412 |
| Critical commercial assays | ||
| 10x Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) | 10x Genomics | Single Cell 3′ v3.1 |
| Deposited data | ||
| Guided differentiation scRNA-seq | This paper | GEO: GSE230587 |
| 16-day differentiation time-course scRNA-seq | Elorbany, et al., 2022 | GEO: GSE175634 |
| Healthy fetal heart tissue scRNA-seq | Miao et al., 2020 | GEO: GSE138979 |
| Day-100 multi-lineage organoid scRNA-seq | Silva et al., 2021 | GEO: GSE153075 |
| Human peripheral blood mononuclear cells (PBMCs) | 10x Genomics | 20k Human PBMCs, 3′ HT v3.1, Chromium X |
| Experimental models: Cell lines | ||
| Human iPSC line | Banovich, et al., 2018 | 19114 |
| Human iPSC line | Banovich, et al., 2018 | 19130 |
| Human iPSC line | Banovich, et al., 2018 | 19152 |
| Software and algorithms | ||
| Cell Ranger v6.1.2 | Zheng, et al., 2017 | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome |
| Vireo v0.5.6 | Huang, et al., 2019 | https://github.com/single-cell-genetics/vireo |
| Seurat v4.3.0 | Stuart, et al., 2019 | https://satijalab.org/seurat/ |
| Tidyverse v2.0.0 | Wickham, et al., 2019 | https://www.tidyverse.org/ |
| scPred v1.9.2 | Alquicira-Hernandez, et al., 2019 | https://github.com/powellgenomicslab/scPred |
| RColorBrewer v1.1-3 | Neuwirth, 2022 | https://cran.r-project.org/web/packages/RColorBrewer/index.html |
| Other | ||
| AggreWell™800 24-well plate | STEMCELL Technologies | Cat.# 34811 |
| Anti-Adherence Rinsing Solution | STEMCELL Technologies | Cat.# 07010 |
| Ultra-low adherent plate | STEMCELL Technologies | Cat.# 100-0083 |
| Wide bore pipette tip | Thermo Fisher Scientific | Cat.# 2079GPK |
| 40μm cell strainer | Bel-Art | Cat.# H136800040 |
| Color schemes | Tol, 2021 | https://personal.sron.nl/~pault/ |