FIG 3.

The Corynebacterium tuberculostearicum pangenome. (A) Anvi’o pangenomic map for 28 C. tuberculostearicum genomes (including 5 NCBI reference genomes). Genomic rings are annotated by skin site and HV (healthy volunteer) metadata and ordered by pyANI average nucleotide identity (ANIb). Genome margins are manually adjusted for clarity. (B) Heap’s Law estimate of pangenome openness for 28 genomes. A rarefaction curve showing the total number of genes accumulated with the addition of new genome sequences in random order with 1000 permutations. Shaded regions represent the 95% confidence interval. A Heap’s law model was fit to the resultant curve to calculate k and γ values (1977±38.0 and 0.30±0.01, respectively). (C) Number of core (belonging to all genomes), accessory (belonging to two or more genomes), and singleton (belonging to only one genome) genes. The expanded pangenome contains 5451 genes using 90% sequence identity as a cutoff parameter.