TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| KS533 | Rough:K1:H7 | 2 |
| DH5α | thi-1 recA hsdR17 lac | A. Francky |
| GE2897 | MC4100 Δ82(himA)::tet | 8 |
| GE3653 | MC4100 lrp-201::Tn10 | 8 |
| MC4100 | araD139Δ(argF-lac)U169 rpsL150 relA1 flbB5301 ptsF25 deoC1 | 16 |
| SBS688 | MC4100 Δcrp39 | 12 |
| RH90 | MC4100 rpoS::Tn10 | R. Hengge-Aronis |
| RH98 | MC4100 relA251::kan spoT207::cat | 16 |
| MC4100 hns::kan | hns | 30 |
| RK5288 | MC4100 fnr-250 zcj-637::Tn | 7 |
| AB1133 | Sensitive to all colicins | B. Bachman |
| BZB2116 | Harboring pColK-K235 | 23 |
| Plasmids | ||
| pColK-JA533 | Colicin K | This study |
| pCB267 | AprlacZ promoter probe vector | 27 |
| pIK32 | pUC19 with the 7-kb Sau3AI fragment encoding cka, cki, ckl | This study |
| pIK100 | pUC19 with the 1-kb EcoRI fragment of pIK32 | This study |
| pIK471 | pCB267 cka-lacZ | This study |
| pUC19 | Apr cloning vector | 31 |