TABLE 3.
Influence of mitomycin C and IHF on cka-lacZ expression
| Time (h) | Expression ina:
|
|||||
|---|---|---|---|---|---|---|
| MC4100
|
Mitomycin Cb
|
himAc or HimA−
|
||||
| OD600 | Units | OD600 | Units | OD600 | Units | |
| 3 | 0.76 | 22 | 0.42 | 60 | 0.52 | 62 |
| 5 | 3.46 | 138 | 2.81 | 343 | 2.92 | 137 |
| 8 | 4.47 | 389 | 3.53 | 807 | 4.23 | 394 |
| 24 | 3.09 | 490 | 1.70 | 980 | 4.28 | 695 |
a
The expression in Miller units is indicated at four different times (during the growth cycle) of cell growth and compared with the expression in strain MC4100.
b
The culture of strain MC4100 was divided into two parts in the early log phase at an OD600 of 0.3. Mitomycin C was added at 0.5 μg/ml to one part. The data shown represent the average of three independent experiments.
c
β-Galactosidase activity (in Miller units) was measured in the himA mutant strain GE2897, which is defective in IHF. The data shown represent the average of two independent experiments.