TABLE 4.
Aerobic and anaerobic regulation of the cka-lacZ fusiona
Strain | Activity under aerobiosis
|
Activity under anaerobiosis
|
||||||
---|---|---|---|---|---|---|---|---|
No induction
|
Induction
|
No induction
|
Induction
|
|||||
OD600 | Units | OD600 | Units | OD600 | Units | OD600 | Units | |
fnr+b | 3.83 | 79 | 2.91 | 225 | 1.59 | 30 | 1.48 | 428 |
fnrc | 4.05 | 40 | 2.49 | 240 | 0.49 | 16 | 0.9 | 280 |
After inoculation, the strains were grown in LB medium aerobically, split into two parts, and further grown aerobically and anaerobically for 1 h, after which they were split again and one part was induced with mitomycin C. β-Galactosidase activity (in Miller units) was assayed 4 h after inoculation. The data represent the average of two independent experiments.
Strain MC4100 with pIK471.
Strain RK5288 with pIK471.