FIG 1.

Construction and growth characteristics of the dnaK mutant in C. sakazakii. (A) Construction of a pCVD442 suicide plasmid for dnaK deletion in C. sakazakii. (B) The growth of wild-type (WT) and ΔdnaK C. sakazakii strains, as well as the complementation of dnaK in the ΔdnaK strain (ΔdnaK-C), was monitored in LB medium at pH 7.0. The optical density at 600 nm (OD₆₀₀) was used to measure the change in bacterial optical density over time, while CFUs were used to determine the viable cell count at different time points. Each data point represents the average value and standard deviation derived from three independent biological replicates.