FIG 3.

Impact of dnaK on acid sensitivity, cell adhesion, and invasion of C. sakazakii. (A) Growth curves of the WT, ΔdnaK mutant strain, and complemented bacteria in LB medium at different pH values were measured. (B) The relative adhesion of HCT-8 cells to the WT, ΔdnaK mutant strain, and complemented strain was assessed. (C) The relative invasion of HCT-8 cells by the WT, ΔnlpD mutant strain, and complemented strain was measured. In the adhesion and invasion experiments, Salmonella enterica was used as a positive control and Escherichia coli DH5α was used as a negative control. All experiment was repeated 3 times; *, indicates significant differences.