FIG 5.

Protein profiling changes caused by dnaK gene knockout in the proteomic analysis. (A) Volcano plot showing the impact of dnaK knockout on protein abundance. The criteria for significant changes are defined as a fold change greater than 2 and a P value of less than 0.05. Red dots represent significantly upregulated proteins, green dots represent significantly downregulated proteins, and gray dots represent proteins without significant differences. The number of proteins in each category is labeled in the figure. See also Table S1 in the supplemental material. (B) Gene Ontology (GO) analysis of the significantly upregulated proteins caused by dnaK knockout in the biological process (BP), cellular component (CC), and molecular function (MF) categories. The size of the shape represents the number of proteins classified in that category, while the color represents the ratio of the number of proteins classified in that category to the total number of proteins in that category. See also Table S2 in the supplemental material. (C) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the significantly upregulated proteins caused by dnaK knockout in metabolic pathways. No significant enrichment was observed in the KEGG analysis for the downregulated proteins. The size of the shape represents the number of proteins classified in that category, while the color represents the ratio of the number of proteins classified in that category to the total number of proteins in that category. See also Table S2.