FIG 6.

Protein deamidation changes caused by dnaK gene knockout in proteomic analysis. (A) The volcano plot illustrates the effect of dnaK knockout on the abundance of deamidated proteins. Significance was determined based on fold change >2 and a P value of <0.05. Proteins that were significantly upregulated are shown in red, while those that were significantly downregulated are shown in green, and those with no significant difference are shown in gray. The number of proteins in each category is indicated in the figure. See also Table S3 in the supplemental material. (B) Gene Ontology (GO) analysis was performed to investigate the enrichment of significantly upregulated deamidated proteins caused by dnaK knockout in biological processes (BP), cellular components (CC), and molecular functions (MF). The size of each shape represents the number of deamidated proteins in that category, while the color represents the proportion of deamidated proteins in that category relative to the total number of proteins in that category. See also Table S4 in the supplemental material.