FIG 7.

Impact of dnaK Deletion on GrpE. (A) Venn diagram depicting the proteins that exhibited altered abundance (blue) or deamidation modification (yellow) upon dnaK deletion. The corresponding data for each protein are presented in the diagram. See also Table S5 in the supplemental material. (B) Amino acid sequence of GrpE protein with deamidation modification sites (in red) identified by proteomics in the dnaK deletion strain. The deamidation modification sites that showed a significant increase in abundance upon dnaK deletion compared with that in the wild type are shaded in gray. (C) Abundance of GrpE protein with deamidation modification at the N(326), N(433), N(467), and N(487) sites upon dnaK deletion. *, indicates a significant difference. (D) Transcriptional levels of the grpEL gene in wild-type, dnaK deletion, and dnaK deletion complementation strains. The experiments were repeated biologically three times. (E and F) Growth curves of wild-type, dnaK deletion, grpEL deletion, and dnaK-grpEL double-deletion strains at 30°C (E) and 37°C (F). The experiments were repeated biologically three times. Ns, indicates no significant difference.