TABLE 1.
Bacterial strains and plasmids used in this study
| Strain and plasmid | Description | Reference or source |
|---|---|---|
| Cronobacter sakazakii | ||
| WT | Wild-type Cronobacter sakazakii BAA-894 | 14 |
| ΔdnaK | Markerless deletion mutant ΔdnaK | This study |
| ΔdnaK-C | dnaK complementation in ΔdnaK | This study |
| ΔgrpEL | Markerless deletion mutant ΔgrpEL | This study |
| ΔgrpEL-C(T) | grpEL temp sensitive complementation in ΔgrpEL | This study |
| ΔdnaK-ΔgrpEL-C(T) | dnaK knockout in ΔgrpEL-C(T) | This study |
| Escherichia coli | ||
| S17 lambda pir | Strain for construction harbouring lambda pir | 20 |
| S17 lambda pir-ΔdnaK | S17 lambda pir harbouring pCVD442-ΔdnaK | This study |
| S17 lambda pir-ΔgrpEL | S17 lambda pir harbouring pCVD442-ΔgrpEL | This study |
| DH5α | Strain for construction | 39 |
| DH5α-grpEL-C(T) | DH5α harbouring pKC1139-grpEL | This study |
| Plasmids | ||
| pACYC184 | Low-copy plasmid | 40 |
| pACYC184-dnaK | dnaK complementation vector | This study |
| pCVD442 | Suicide plasmid for markerless deletion | 36 |
| pCVD442-ΔdnaK | dnaK deletion plasmid | This study |
| pKC1139 | Temp sensitive carrier | 41 |
| pKC1139-grpEL | grpEL temp sensitive complementation vector | This study |