TABLE 2.

Primers used in this study

Primer name by use	Sequence (5′–3′)
For construction
pCVD442-fwd	GGCTGTCAGACCAAGTTTACTCATATATACTTTAGATTG
pCVD442-rev	GCAGATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG
ΔdnaK-A	GAAAAAGGAAGAGTATCTGCGGTACGGTACGGCCATACTGTTCG
ΔdnaK-B	GCGATTAACCCATCTAAACGTCTCCACTAAAAAATCGTCATC
ΔdnaK-C	CGTTTAGATGGGTTAATCGCCCTGATGCAGGGTAGATAAC
ΔdnaK-D	GATTAATTGTCAAGGCTAGCGTGCTGTTTCACCTGCACCTGAAC
dnaK-comp-fwd	GGTCTAGAGCCGTCTATGATCCCGGAGG
dnaK-comp-rev	GGTCTAGATACCCTGCATCAGGGCGATTA
ΔgrpEL-A	GAAAAAGGAAGAGTATACCTGATGCTGATGCAGCAG
ΔgrpEL-B	TACGTTCTTTAGCTGCCATATCGTTATTTC
ΔgrpEL-C	GATATGGCAGCTAAAGACGTAGGTATGATGTAATTTAATCTTCATAC
ΔgrpEL-D	GATTAATTGTCAAGGCTGCGTTAAAATCGTAGGGCGGGT
pACYC184-fwd	GAATTCGTAATCATGTCATAGCTGT
pACYC184-rev	GGATCCTCTAGAGTCGACCTGCA
grpEL-comp-fwd	TGCAGGTCGACTCTAGAGGATCCGAAATTGAATCTAAATCTGCTG
grpEL-comp-rev	ACAGCTATGACATGATTACGAATTCCCTGAAGTATGAAGATTAAATTCACAT
For sequencing  confirmation
ΔdnaK-E	GTGGCACGACGTCGGGTAAATC
ΔdnaK-F	GTGGCCGCCATCGCAAAGTTGATC
ΔgrpEL-E	CTTTATTCAGATGGCGCATG
ΔgrpEL-E	CCTGTAGGGCGGGTAAGCGAAGC