FIG 2.

(A and B) Two-channel maximum intensity z-projection of representative Airyscan-processed z-stacks of immunofluorescence-stained M. thermautotrophicus ΔH strains (A) and scanning electron micrographs of the same three untreated M. thermautotrophicus ΔH strains (B). (A) For the Airyscan process z-stacks, DAPI staining is shown in magenta. The Alexa Fluor 488 conjugated antibody, which is attached to the primary anti-Mth60-fimbria antibody, is depicted in green. Orange arrows indicate Mth60-fimbria structures, and the 1-μm scale bar represents the size for all pictures because the magnification is the same. Three M. thermautotrophicus strains were analyzed: M. thermautotrophicus ΔH wild-type (wild-type), M. thermautotrophicus ΔH containing a shuttle vector for constitutive expression of the Mth60 fimbria-encoding operons (constitutive expression), and M. thermautotrophicus ΔH with a deletion of the Mth60 fimbria-encoding operons (deletion). (B) The scale bar indicates 1 μm for all three images for the scanning electron micrographs. The same strains of M. thermautotrophicus ΔH as in panel A were analyzed and are depicted in the same order.