TABLE 1.
Primer list
| Name | Purpose | Sequence | Reference or source |
|---|---|---|---|
| Seq_CF1 | Analysis genome integration Δmth58-61 | CCACCAGTTCGACTCCCTGG | 32 |
| Seq_CF2 | Analysis genome integration Δmth58-61 | CTGTTAAAGGCGGGGGTGG | 32 |
| Seq_CF3 | Analysis genome integration Δmth58-61 | CTTGGGTGATGATGGGATGTATTG | 32 |
| Seq_CF4 | Analysis genome integration Δmth58-61 | CGAGGAGAAACACATCCAGCTG | 32 |
| Seq_CF5 | Analysis pMVS1111A: PhmtB-mth58-61 | GTTAATCCAGCACATCCTCC | 32 |
| Seq_CF6 | Analysis pMVS1111A: PhmtB-mth58-61 | CCTGTCCAACTTATACCTTTGG | 32 |
| Gib_CF15 | Cloning pCF702 | CGGCTCTAGCTATGTCCGATC | 32 |
| M13 FW | Cloning pCF702 | TGTAAAACGACGGCCAGT | Eurofins genomics (Konstanz, Germany) standard primer list |
| Res_CF3 | Cloning pCF702 | TAGCCTATTCGGCGCGCCGAAATCCCAACCTTCATATAATATTGCAAG | This study |
| Res_CF4 | Cloning pCF702 | TCATGGCTACGTCGACCGGGCCATAACACATACCAC | This study |
| Res_CF5 | Cloning pCF702 | TCAGGATTCGGGCCGGCCTCCGTAAGAAGGGAATTGAACCTCC | This study |
| Res_CF6 | Cloning pCF702 | CGTTGCAATGCATATGGGTACTGTGTGAGGGTCATATTCTG | This study |
| Res_CF7 | Cloning pCF702 | TATTGCAATGGTCGACAGTGGGCAAGTTGAAAAATTCAC | This study |
| Res_CF8 | Cloning pMVS1111A: PhmtB-mth58-61 | ATGCCATTACGGCGCGCCTCACCACACTGTGGCTCCG | This study |
| Res_CF9 | Cloning pCF702 | TACGTTGCCAGGCCGGCCCCCATGAACCAACCGATGGC | this study |
| Res_CF10 | Cloning pMVS1111A: PhmtB-mth58-61 | CGTTGATCCGTTAATTAAGTCCAGGAATAAGGAAGACATCCCG | This study |
| Seq_CF9 | Analysis genome integration Δmth58-61 | GTGGTCCGCTGGCAGATATG | This study |
| Seq_CF10 | Analysis genome integration Δmth58-61 | GCTGCTTCCCTGAGGATGTC | This study |
| Seq_CF11 | Analysis genome integration Δmth58-61 | CTGCTCCCTGGGATGCCTTG | This study |
| Seq_CF12 | Analysis genome integration Δmth58-61 | GTAATCCCGCTTATGGTTGCCC | This study |