TABLE 1.
Primers and probes designed in this study to quantify ToBRFV Mo and RdRP genes
| Primer or probe | Description | Sequence (5′ to 3′) | Amplicon length (bp) or modificationsa |
|---|---|---|---|
| Primers | |||
| ToBRFV_Mo_F | ToBRFV Mo gene; forward primer | TCA GTG TCT GTT TGG TCG ATA A | 105 |
| ToBRFV_Mo_R | ToBRFV Mo gene; reverse primer | GGA ACG ACT TTG AAC TGA AAC C | |
| ToBRFV_RdRP_F | ToBRFV RdRP gene; forward primer | AGC CAC AAG AGA TAA TGT TCG TA | 103 |
| ToBRFV_RdRP_R | ToBRFV RdRP gene; reverse primer | ACA TCA GAC CTT CGT CGA TAA AT | |
| Probes | |||
| ToBRFV_Mo_P | ToBRFV Mo gene; probe | AGA GCG GAC GAG GCA ACT CTT G | FAM/ZEN/IBHQ |
| ToBRFV_RdRP_P | ToBRFV RdRP gene; probe | ACG GTA AAG GAA CAC GCT GTC AGT | FAM/ZEN/IBHQ |
a
FAM, 6-carboxyfluorescein; ZEN, proprietary to IDT; IBHQ, 3’-Iowa Black Fluorescent Quencher.