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. Author manuscript; available in PMC: 2023 Jul 26.
Published in final edited form as: Sci Transl Med. 2021 Jul 28;13(604):eabj2138. doi: 10.1126/scitranslmed.abj2138

Fig. 2. Inhibition of ACE increased susceptibility to bacterial infection in vivo.

Fig. 2.

(A) WT and ACE KO mice were infected with MRSA intravenously (8 × 107 CFU/100 μl). The bacterial concentration in the blood is shown at 24 hours after infection. (B to D) Bacterial count in spleen (B), liver (C), and lung (D) was determined at 48 hours after infection. Indicated groups of mice were treated with either ramipril (Ram; 40 mg/liter) or losartan (Los; 600 mg/liter) for 1 week before infection. Drugs were administered throughout the study. Neutrophil depletion (Dep) was achieved by daily injection of mice with a murine anti-PMN antibody, starting 24 hours before infection with MRSA to the end of study. Mice were perfused with PBS before harvesting tissues. Two-way ANOVA with Bonferroni’s correction for multiple comparisons was used to analyze group comparisons, and data are presented as means ± SEM (n = 7 mice per group). ***P < 0.001 and ****P < 0.0001.