Figure 8.

Construction of risk model and functional validation of critical genes. (A) Differential analysis between clust1 and no_clust1 in TCGA dataset; (B) Differential analysis between clust2 and no_clust2 in TCGA dataset; (C) Differential analysis between clust3 and no_clust3 in TCGA dataset; (D) A total of 961 promising candidates were identified among the differentially expressed genes; (E) Trajectory of each independent variable as lambda changes; (F) Confidence interval under lambda; (G) Coefficients of prognostic-related genes obtained from multivariate Cox analysis; (H) Western blot assay of DUSP1 protein expression in 786-O cell after transfection of Si-DUSP1; (I) Colony formation assay was carried out to evaluate the proliferation of 786-O cell; (J, K) Transwell assay was used to assess the migration and invasion of 786-O cell. (L) Western blot assay of p21 and p53 protein expression in 786-O cell after transfection of Si-DUSP1. *, p< 0.05; (M) SA-β-gal staining of 786-O cell. *, p< 0.05.