TABLE 1.

Effect of energy inhibitors on chromate uptake by everted membrane vesicles

Treatment	Initial rate of chromate uptake (nmol of 51CrO42−/mg of protein/min)a
+NADH	1.59 ± 0.10
No NADH	0.52 ± 0.06
+4 mM NaCN	0.34 ± 0.20
+50 μM HQNO	0.54 ± 0.26
+20 mM NaN3	0.60 ± 0.26
+50 μM Carbonyl cyanide m-chlorophenylhydrazone	0.66 ± 0.10
+5 μM Nigericin	0.55 ± 0.20
+1 mM Na2SO4	0.48 ± 0.13

^aChromate uptake by vesicles from PAO1(pCRO616) was assayed in phosphate buffer with 2 mM NADH and 400 μM chromate, as described in the text. The values are the mean ± standard deviation of three assays in duplicate.