Fig. 5. Trajectory analysis of NK cells.

(A) Trajectory of NK cells: colored by cluster membership (left) and pseudotime (right). The red arrows indicate the root nodes for the pre- and post-infusion trajectories (see Materials and Methods) relative to which pseudotime is computed. (B) Boxplot of pseudotime of NK clusters N5 and N6 that form the post-infusion trajectory; P value reported was computed using Wilcoxon rank sum test. (C) Trajectory plots split by NK cell product. (D) Violin plots showing the distribution pseudotime in pre- and post-infusion NK cells by groups. Differences in pseudo-time between groups in both pre- and post-infusion cells was significantly different [analysis of variance (ANOVA), P < 2 × 10⁻¹⁶ by a pairwise Tukey’s test reported in fig. S8]. (E) Hallmark pathways enriched in genes overexpressed in the low and high N5 cells (P < 0.05 and q < 0.1, respectively).