Fig. 2. Characteristics of ScfDNA.
ScfDNA insert size profile with multiple peaks and valleys using A. Double-stranded library preparation (Peach solid line) and single-stranded library preparation (Turquoise solid line). B. Single-stranded library preparation (Turquoise solid line) and Nick repair enzyme-treated ScfDNA (Turquoise dashed line). C. Double-stranded library preparation (Peach solid line) and nick repair enzyme-treated ScfDNA (Peach dashed line). D. Human and microbial origins of salivary cell-free DNA. Percentage of reads of ScfDNA mapping to humans and microbes. E. Genomic element analysis using different library preparation methodologies is comparable to each other.