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[Preprint]. 2023 Jul 10:rs.3.rs-3126389. [Version 1] doi: 10.21203/rs.3.rs-3126389/v1

Table 3:

Guaranteed production rates for succinate (Rategrnt) in iJR904 in CM2 medium for succinate production

Number of knockouts Deleted reaction Biomass formation rate (h−1) Production rate (mmol.gDW−1.h−1) SoGC (h−1) Deleted genes Co-knockout reactions
min max
Single ADHEr 0.16 5.11 9.50 1.41 b1241 LCADi_copy2
Double ADHEr, LDH_D 0.15 8.08 9.51 1.43 b1241, b2133, b1380 LCADi_copy2
Triple ADHEr, LDH_D, PFL 0.12 11.08 12.73 1.53 b1241, b2133, b1380, b3114, b0902, b3951 LCADi_copy, OBTFL
Quadruple ADHEr, LDH_D, PFL, THD2 0.11 12.29 13.01 2.58 b1241, b2133, b1380, b3114, b0902, b3951, b1602 LCADi_copy, OBTFL
Quintuple ADHEr, LDH_D, GLUDy, PFL, THD2 0.10 12.34 13.06 2.61 b1241, b2133, b1380, b1761, b3114, b0902, b3951, b1602 LCADi_copy, OBTFL
Table 3–1: Maximized production rates for succinate (Rate max ) in iJR904 in rich medium (LB) for succinate production
Number of knockouts Deleted reaction Biomass formation rate (1/h) Production rate (mmol*gDW−1*hr−1) Deleted genes Co-Knockout reactions
Single ADHEr 1.35 20.10 b1241 LCADi_copy2
Double F6PA, PFK 1.28 33.69 b0825, b3946, b3916, b1723 -
Triple ACKr, GLCpts, PYK 0.56 54.88 b2296, b3115, b1849, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 DHAPT, GART, PPAKr
Quadruple ACKr, ARGDC, GLCpts, PYK 0.56 64.72 b2296, b3115, b1849, b2938, b4117, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 GART, PPAKr, DHAPTs
Table 3–2: Maximized production rates for succinate (Rate max ) in iML1515 in CM2 medium for succinate production
Number of knockouts Deleted reaction Biomass formation rate (1/h) Production rate (mmol*gDW−1*hr−1) Deleted genes Co-Knockout reactions
Single ATPS4rpp 0.25 12.73 b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739 -
Double ATPS4rpp, PGL 0.24 16.54 b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739, b0767 -
Triple PGI, ATPS4rpp, G6PDH2r 0.17 23.16 b4025, b3734, b3733, b3736, b3732, b3737, b3731, b3738, b3739, b3735, b1852 -
Quadruple PFL, ACALD, THD2pp, THD2pp 0.19 23.49 b0351, b1241, b0903, b3951, b2579, b3952, b3114, b0902, b1602, b2913 OBTFL, ‘ALCD2x’, ‘ALCD19’
Table 3–3: Maximized production rates for succinate (Ratemax) in iML1515 in rich medium (LB) for succinate production
Number of knockouts Deleted reaction Biomass formation rate (1/h) Production rate (mmol*gDW−1*hr−1) Deleted genes Co-Knockout reactions
Single ARGDC 1.08 19.72 b4117 -
Double ARGDC, FADRx 1.05 22.09 b4117, b3844 FADRx, FE3Ri, FLVRx
Triple NDPK5, ASPTA, ARGDC 1.03 28.14 b0474, b2518, b0928, b4054, b4117 ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, TYRTA, PHETA1, LEUTAi
Quadruple NDPK5, PFL, LDH_D, ACALD 0.75 40.97 b0474, b2518, b2579, b3952, b0902, b3951, b0903, b3114, b1380, b0351, b1241 ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, OBTFL, ALCD2x, ALCD19