Table 4:
The best solutions based on the desired evaluation indexes for succinate production under anaerobic condition in iJR904
| Evaluation index | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| SSP | linearMOMA | SoGC | |||||||
| Number of knocked out reactions | Best knockout strategy | FBA biomass (h−1) | FBA succinate rate (mmol.gDW−1.h−1) | Biomass * succinate rate (mmol.gDW−1.h−1) | Best knockout strategy | MOMA biomass (h−1) | MOMA Succinate rate (mmol.gDW−1.h−1) | Biomass * succinate rate (mmol.gDW−1.h−1) | Best knocko strateg |
| 1 | ADHEr | 0.16 | 0.83 | 0.14 | ADHEr | 0.03 | 2.38 | 0.08 | ADHEr |
| 2 | ADHEr, LDH_D | 0.16 | 8.73 | 1.43 | ADHEr, ATPS4r | 0.12 | 8.32 | 1.01 | ADHEr LDH_D |
| 3 | ADHEr, LDH_D, PFL | 0.12 | 12.24 | 1.53 | ADHEr, ATPS4r, RPE | 0.13 | 8.60 | 1.19 | ADHEr ATPS4 LDH_D |
| 4 | ADHEr, LDH_D, PFL, URIK2 | 0.12 | 12.24 | 1.53 | ADHEr, ATPS4r, LDH_D, RPE | 0.13 | 8.71 | 1.20 | ADHEr LDH_D HEX1, THD2 |
| 5 | ADHEr, P, PFL, SUCOAS, RNDR3 | 0.12 | 12.25 | 1.54 | ADHEr, ATPS4r, GLYK, F6PA, RPE | 0.14 | 8.63 | 1.23 | ADHEr LDH_D HEX1, THD2, DRPA |