Extended Data Figure 4. Characterization of ecDNA evoluationary model across various initial conditions.

(a) Representative joint ecDNA copy-number distributions across varying levels of co-segregation and co-selection. Co-occurrence frequency and Pearson’s correlation are reported for each joint distribution. (b-d) Average frequency of cells carrying both ecDNA species and Pearson’s correlation of ecDNA copy-numbers in single cells are reported across simulations of 10 replicates of 1 million cells for varying initial ecDNA copy-numbers: (b) 1 copy of each ecDNA species; (c) 10 copies of each ecDNA species; (d) 100 copies of each ecDNA species. Selection acting on cells with only one but not both ecDNA species is maintained at 0.2 and selection acting on cells without either ecDNA is maintained at 0.0 for all simulations.