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. 2023 Jun 22;8(12):e160345. doi: 10.1172/jci.insight.160345

Figure 4. Splenic T cells have higher metabolic activity following ischemic AKI.

Figure 4

(A) Unbiased UMAP analysis of concatenated flow cytometry data of splenic T cells from control mice (gray and black), mice during renal ischemia (red), and post-renal IRI 4 hours (blue) and 48 hours (purple) mice. Multiple enzymes associated with glycolysis, fatty acid oxidation, and oxidative phosphorylation drove the separation of splenic T cells, showing that metabolically activated T cell subsets were increased in the spleens following renal IRI (arrows). (B) Histograms comparing splenic T cells from control mice (gray) and post-IRI mice (48 hours) (purple). (C) Normalized MFI of significantly changed metabolic markers on splenic CD4+, CD8+, and DN T cells. Statistical analyses were performed using 2-way ANOVA followed by Tukey’s post hoc analysis (n = 10 mice in each group). Data are from 2 independent experiments. *P < 0.05, compared with the control group; †P < 0.05, compared with the renal ischemia group; P < 0.05, compared with the post-renal IRI 4 hours group. AKI, acute kidney injury; CPT1a, carnitine palmitoyltransferase 1a; DN, double-negative; IRI, ischemia/reperfusion injury; UMAP, uniform manifold approximation and projection.