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. 2023 Jul 10;8(13):e167329. doi: 10.1172/jci.insight.167329

Figure 3. TLR7 is upstream of IRF5.

Figure 3

(A) Graph shows TLR7 mRNA levels in CD4+ T cells from ART HIV-1+, EC, and HIVfree individuals analyzed by qPCR. The Kruskal-Wallis followed by the Dunn’s multiple-comparison tests were used for significance. *P < 0.05, **P < 0.01. n =9. CD4+ T cells purified from ART HIV-1+, EC, and HIVfree donors were treated in vitro with imiquimod (IMQ) or medium alone for 30 hours. (B–D) Graphs represent (B) the percentage of IRF-5+, (C) the percentage of apoptotic cells, and (D) the percentage dead memory CD4+ T cells. Data are presented as the mean ± SD. The Friedman’s followed by the Dunn’s multiple-comparison tests were used to determine statistical differences. *P < 0.05, **P < 0.01. n = 6. (E and F) Graphs show correlations between (E) TLR7 and IRF-5 expression and (F) annexin V and TLR7 expression in ART HIV-1+ donors. The Spearman’s r test was used for statistical significance. n =9.