FIG. 1.

Inhibitory effects of CI-959-A and MAbs to CSMs on SPL for patients with HAM/TSP. PBMCs were donated by 15 patients with HAM/TSP, and 7.5 × 10⁴ cells were cultured for 6 days in the presence of 10 μg of MAbs per ml or a 1-μM concentration of compound. The proliferation of T cells in the absence of test materials was 14,314 ± 7,255 cpm (mean ± standard deviation for 15 individuals; the individual titers were as follows: 27,368, 8,512, 8,585, 10,864, 4,154, 24,777, 11,593, 15,348, 10,636, 16,215, 10,723, 25,308, 5,256, 20,074, and 15,301 cpm). A mixture of normal mouse IgG subclasses (IgG1, Ig2a, Ig2b, and Ig3) was used as control antibody. ∗, P < 0.001. The inset shows a dose-response curve for CI-959-A on SPL for patients with HAM/TSP. Data for three representative patients were used, and [³H]thymidine uptake by T cells without the compound was determined to be 100%. The mean ± standard deviation for three patients is shown.