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Inhibition of functional DC production by CI-959-A. Various numbers of PBMCs were cultured in a 96-well plate in the presence of 1 μM CI-959-A or CI-959 to produce mature DCs. After 7 days, the DCs were treated with mitomycin C, washed, and used for T-cell stimulation. Allogeneic, unseparated PBMCs (5 × 104/well) were used as responders. Data represent those from three separate experiments.
