TABLE 1.
Inhibitory effects of CI-959 and CI-959-A on primary proliferative response of T cells
| Compound | EC50
(μM)a
|
||||
|---|---|---|---|---|---|
| PHA | Allogeneic PBMCs | Allogeneic cultured DCs | Autologous EBV-infected B cells | Autologous HTLV-I-infected T cells | |
| CI-959 | >4.0 | >4.0 | >4.0 | >4.0 | >4.0 |
| CI-959-A | 0.51 ± 0.24 | 0.52 ± 0.04 | 0.65 ± 0.10 | 0.52 ± 0.01 | 0.62 ± 0.05 |
a
The EC50s of CI-959 and CI-959-A were determined by quantification of the inhibition of the proliferative response of T cells to various stimulators. The proliferative response of unseparated PBMCs (5 × 104/well) in the absence of compounds was 156,274 ± 112,391 cpm for PHA (2 μg/ml), 11,937 ± 3,514 cpm for allogeneic PBMCs (5 × 104/well), 14,771 ± 10,026 cpm for allogeneic cultured DCs (5 × 103/well), 28,165 ± 11,205 cpm for autologous EBV-infected B cells (3 × 104/well), and 30,306 ± 19,772 cpm for autologous HTLV-I-infected T cells (5 × 104/well). Values are means ± standard deviations for three to six independent donors.