TABLE 3.
Inhibitory effects of CI-959 and CI-959-A on differentiation of DCs from PBMCsa
| Compound | Concn (μM) | % Differentiation of
DCs
|
|
|---|---|---|---|
| CD83− DCs | CD83+ DCs | ||
| None | 0 | 7.30 ± 3.50 | 2.70 ± 1.11 |
| CI-959 | 1 | 6.41 ± 1.30 | 2.50 ± 0.40 |
| CI-959-A | 0.5 | 3.90 ± 1.31b | NDc |
| 1.0 | 2.25 ± 0.61de | 1.20 ± 0.65be | |
a
The effect of CI-959-A on differentiation of DCs from PBMCs was determined. CD83− DCs were differentiated by culturing PBMCs in the presence of GM-CSF and IL-4 for 7 days, and CD83+ DCs were differentiated by using GM-CSF, IL-4, and TNF-α. In each experiment, five independent cultures were used. The DC differentiation rate was calculated as follows: 100 × (number of DCs differentiated/number of PBMCs used). Means ± standard deviations are shown.
b
P < 0.05 compared with no compound.
c
ND, not determined.
d
P < 0.01 compared with no compound.
e
P < 0.05 compared with CI-959.