FIG. 5.

Representative Western blot of S48-3-13- and S48-3-17-negative A. baumannii clinical isolates after SDS-PAGE of the proteinase K-treated bacterial lysates (20 μl each), transfer onto a PVDF membrane, hydrolysis at 100°C for 1 h in 0.1 M HCl, and immunostaining with lipid A-specific MAb S1. Bacteria are, from left to right, strain 42 (lane A), strain GNU 1086 (lane B), strain NCTC 7844 (lane C), strain 36 (lane D), strain RUH 1205 (lane E), strain RUH 3204 (lane F), strain RUH 1486 (lane G), strain RUH 2180 (lane H), strain 10086 (lane I), and strain RUH 1907 (lane J).