TABLE 1.
Reactivities of MAbs S48-3-13 and S48-3-17 in dot and Western blots with LPSs from proteinase K-treated bacterial whole-cell lysates from A. baumannii clinical isolates investigated in this study and O-banding patterns obtained following acid hydrolysis of membrane-bound LPSs and immunostaining with MAb S1 of proteinase K-treated bacterial lysates from A. baumannii strains which did not react with MAb S48-3-13 or MAb S48-3-17
| Straina | Sourceb | City (country)c | Positiond | Reactivity
with MAbe
|
Pattern obtained after acid hydrolysisf | |
|---|---|---|---|---|---|---|
| S48-3-13 | S48-3-17 | |||||
| ATCC 17904 | Urine | Not known | E4 | − | − | − |
| ATCC 19606T | Urine | Not known | E3 | − | − | − |
| NCTC 7844 | Not known | Not known | G8 | − | − | C |
| 9g | Sputum | Malmö (SE) | G7 | − | − | − |
| 10g | Wound | Rotterdam (NL) | G5 | − | + | ND |
| 11g | Wound | Rotterdam (NL) | G2 | − | + | ND |
| 12g | Toe web | Rotterdam (NL) | E6 | − | + | ND |
| 13g (I) | Bronchus | Nijmegen (NL) | F3 | + | − | ND |
| 14g | Not known | Not known | F1 | − | − | − |
| 16g | Urine | Rotterdam (NL) | E12 | − | + | ND |
| 17g | Wound | Rotterdam (NL) | G1 | − | + | ND |
| 18g | Urine | Rotterdam (NL) | G3 | − | + | ND |
| 19g | Pus | Leiden (NL) | G6 | − | − | A |
| 20g | Sputum | Rotterdam (NL) | F7 | − | + | ND |
| 21g | Wound | Malmö (SE) | E5 | − | − | − |
| 22g | Urine | Dordrecht (NL) | E9 | + | − | ND |
| 23g | Drain | Rotterdam (NL) | F6 | − | + | ND |
| 24g • | Urine | Rotterdam (NL) | NI | + | − | ND |
| 26g | Wound | Nijmegen (NL) | E11 | + | ND | |
| 27g | Sputum | Dordrecht (NL) | F8 | + | ND | |
| 28g | Urine | Dordrecht (NL) | E10 | + | − | ND |
| 29g (I) | Sputum | Utrecht (NL) | F2 | + | − | ND |
| 30g | Urine | Rotterdam (NL) | F4 | − | − | B |
| 31g (II) | Urine | Rotterdam (NL) | F11 | − | + | ND |
| 32g | Blood | Dordrecht (NL) | F9 | + | − | ND |
| 33g (I) | Urine | Dordrecht (NL) | C12 | + | − | ND |
| 34g • | Urine | Rotterdam (NL) | NI | − | + | ND |
| 36g | Sputum | Rotterdam (NL) | D12 | − | − | D |
| 37g | Blood | Rotterdam (NL) | E2 | − | + | ND |
| 38g | Sputum | Rotterdam (NL) | F10 | − | + | ND |
| 39g | Sputum | Rotterdam (NL) | F12 | − | + | ND |
| 40g | Sputum | Rotterdam (NL) | G4 | − | + | ND |
| 41g | Catheter tip | Utrecht (NL) | E1 | + | − | ND |
| 42g | Ear | Leiden (NL) | F5 | − | − | A |
| RUH 508h | Bronchus | Nijmegen (NL) | D9 | + | − | ND |
| RUH 513h | Bronchus | Nijmegen (NL) | D10 | + | − | ND |
| RUH 733h | Bronchus | Nijmegen (NL) | D11 | + | − | ND |
| RUH 735h | Bronchus | Nijmegen (NL) | D8 | − | − | − |
| RUH 937h | Urine | Nijmegen (NL) | D4 | + | − | ND |
| RUH 975h | Pus | Nijmegen (NL) | D5 | + | − | ND |
| RUH 1027h | Urine | Nijmegen (NL) | D6 | + | − | ND |
| RUH 1202h | Bronchus | Nijmegen (NL) | D7 | + | − | ND |
| RUH 1205h | Bronchus | Nijmegen (NL) | D3 | − | − | E |
| RUH 1277h | Bronchus | Nijmegen (NL) | D2 | + | − | ND |
| RUH 3204i | Tube | Nijmegen (NL) | D1 | − | − | F |
| RUH 1093j | Sputum | Rotterdam (NL) | C6 | − | − | D |
| RUH 1486j | Navel | Rotterdam (NL) | C7 | − | − | G |
| RUH 1752j (ug) | Bronchus | Enschede (NL) | C8 | − | − | D |
| RUH 1907j | Bronchus | Rotterdam (NL) | C9 | − | − | J |
| RUH 2180j | Sputum | Nijmegen (NL) | C10 | − | − | H |
| RUH 2688j | Pharynx | Rotterdam (NL) | C11 | − | − | ND |
| GNU 1078j (I) | Rectal mucosa | Leuven (BE) | B10 | + | − | ND |
| GNU 1079j (I) | Tracheal site | Salford (UK) | B12 | + | − | ND |
| GNU 1080j | Catheter urine | Salisbury (UK) | C1 | − | + | ND |
| GNU 1081j (ug) | Tracheal aspirate | Cork (IE) | C2 | − | − | − |
| GNU 1083j (I) | Urine | London (UK) | C3 | + | − | ND |
| GNU 1084j (I) | Burn wound | Sheffield (UK) | C4 | + | − | ND |
| GNU 1086j (II) | Respiratory tract | Newcastle (UK) | C5 | − | − | B |
| MB 142j | Skin | London (UK) | B7 | − | − | − |
| SJH 9/MB 264j | Skin | London (UK) | B8 | − | − | − |
| SJH 26/MB 288j | Nail fold | London (UK) | B9 | − | − | J |
| 189k | Crural ulcer | Odense (DK) | B6 | − | + | ND |
| 9771k | Urine | Næstved (DK) | B11 | − | − | G |
| 10074k | Urine | Vejle (DK) | A1 | − | − | − |
| 10086k | Urine | Vejle (DK) | A2 | − | − | I |
| 2032l | Sputum | Venlo (NL) | A3 | + | − | ND |
| 2033l | Sputum | Venlo (NL) | A4 | + | − | ND |
| 2034l | Urine | Venlo (NL) | A5 | + | − | ND |
| 2036l | Pus | Venlo (NL) | A6 | + | − | ND |
| 2037l (I) | Sputum | Venlo (NL) | A7 | + | − | ND |
| 3242l (I) | Burn wound | Basildon (UK) | A8 | + | − | ND |
| 3344l | Burn wound | Basildon (UK) | A9 | + | − | ND |
| 3347l | Burn wound | Basildon (UK) | A10 | + | − | ND |
| 3348l | Burn wound | Basildon (UK) | A11 | + | − | ND |
| 3349l | Burn wound | Basildon (UK) | A12 | + | − | ND |
| 3370l | Respiratory tract | Newcastle (UK) | B1 | − | − | B |
| 3371l | Respiratory tract | Newcastle (UK) | B2 | − | − | B |
| 3372l | Respiratory tract | Newcastle (UK) | B3 | − | − | B |
| 3373l | Respiratory tract | Newcastle (UK) | B4 | − | − | B |
| 3374l | Respiratory tract | Newcastle (UK) | B5 | − | − | B |
| 133m | Wound | Malmö (SE) | E7 | − | − | − |
| 147m | Wound | Malmö (SE) | E8 | − | − | − |
ATCC, American Type Culture Collection, Rockville, Md.; NCTC, National Collection of Type Cultures, London, United Kingdom; T, type strain; (I) and (II), outbreak strains belonging to clonal groups I and II, respectively (7); (ug), outbreak strains which could not be allocated to a particular clonal group (7); •, strains used to prepare monoclonal antibodies.
Source or specimen from which the strain was originally isolated.
City and country where the strain was originally isolated. SE, Sweden; NL, The Netherlands; BE, Belgium; UK, United Kingdom; IE, Ireland; DK, Denmark.
Position of the bacterial strains in dot blots (see also Fig. 4); NI, not included (homologous antigen).
Dot blot assays were performed with proteinase K-digested whole-cell bacterial lysates (see Materials and Methods). +, positive reaction; −, no reaction.
A to J, O-antigen patterns; −, no banding pattern observed; ND, not determined.
Dijkshoorn et al. (9).
Dijkshoorn et al. (10).
Horrevorts et al. (19).
Dijkshoorn et al. (7).
Gerner-Smidt and Tjernberg (12).
Dijkshoorn et al. (8).
Tjernberg and Ursing (42).