Table 1.
Background information about the isolates with high and low MIC values in this study.a,b
| GenBank accession number | Strain ID | Initial MIC (μg/mL) | Clade | Subclade | Sample of origin | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Type strain | Enrofloxacin | Lincomycin | Tiamulin | Tilmicosin | Tylosin | Tylvalosin | ||||
| CP042295 | ATCC BAA-2147 | 0.312 | 1.000 | 0.312 | ≤0.250 | ≤0.250 | ≤0.250 | C | 3C | Phallus lymph |
| Field isolates with low MICs | ||||||||||
| SRX9772795 | MycAv 65 | 1.250 | 2.000 | 0.312 | ≤0.250 | ≤0.250 | ≤0.250 | C | 4C | Phallus lymph |
| SRX9772793 | MycAv 63 | 0.625 | 0.500 | 0.156 | 64.000 | 4.000 | ≤0.250 | C | 6C | Tracheal swab |
| SRX7583695 | MycAv 668 | 0.312 | ≤0.250 | 0.156 | ≤0.250 | ≤0.250 | ≤0.250 | C | 6C | Cloacal swab |
| SRX7583629 | MycAv 55 | 2.500 | 1.000 | 0.156 | ≤0.250 | ≤0.250 | ≤0.250 | C | 4C | Ovarian follicles |
| SRX7583693 | MycAv 50 | 10.000 | 4.000 | 0.078 | 0.500 | 0.500 | ≤0.250 | B | Phallus | |
| Field isolates with high MICs | ||||||||||
| SRX7583639 | MycAv 70 | 5.000 | 2.000 | 0.625 | >64.000 | 4.000 | ≤0.250 | B | Phallus lymph | |
| SRX7583671 | MycAv 47 | 5.000 | >128.000 | 0.625 | >64.000 | 4.000 | 1.000 | C | 6C | Lung, air sac |
| SRX7583648 | MycAv 91 | 5.000 | 8.000 | 0.625 | ≤0.250 | ≤0.250 | ≤0.250 | C | 3C | Phallus |
| SRX7583635 | MycAv 67 | 5.000 | >128.000 | 0.625 | >128.000 | >128.000 | 4.000 | C | 1C | Phallus lymph |
| SRX7583636 | MycAv 68 | 2.500 | >128.000 | 0.625 | >128.000 | >128.000 | 4.000 | C | 1C | Phallus lymph |
| NZ_CP041664.1 | MycAv 177 | 2.500 | 2.000 | 0.625 | >128.000 | >64.000 | 4.000 | B | Phallus | |
Field isolates with low MICs are the parent strains of the in vitro cultivated mutants.
aIsolates with initial MIC values highlighted in bold were submitted to efflux pump inhibition tests with the given antibiotics.
bThe classification to different clades and subclades are according to Grózner et al. (2021).