Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
qPCR program
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Pre-Incubation | 95°C | 2 min | 1 |
| Denaturation | 95°C | 10 s | 1 |
| Annealing and Extension | 60°C | 20 s | 39 |
| Melting Curve | 70°C | 5 s | 1 |
| Melting Curve | Increase continuously to 95°C | 5 min | Measurement fluorescence every 0.1°C |
| Hold | 4°C | Forever | |
