Table 8.
PCR mix
| Reagent | Amount |
|---|---|
| 10× La Mix | 5 μL |
| dNTPs | 4 μL |
| Primer 1 | 2 μL |
| Primer 2 | 2 μL |
| Template DNA | 2 μL |
| La Taq | 0.4 μL |
| ddH2O | 34.4 μL |
| Total | 50 μL |
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