REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Competent cell DH5 alpha	TSINGKE	TSC-C01
Chemicals, peptides, and recombinant proteins
Matrigel	Corning	354277
DMEM/F-12	Gibco	C11330500BT
mTeSR1/5× Supplement	STEMCELL	85851
EDTA	Sigma	ED-500G
NaOH	Sigma	V800383
Tris	Solarbio	77-86-1
Na2EDTA·2H2O	Sigma	E5134
Acetic acid	Sigma	A6283
Accutase	Sigma	A6964
Fetal bovine serum	RMBIO	FBS-AB-5XM
Rock inhibitor Y-27632	CHEMCD	C14357
DPBS	GlgBio	EH80028
Ampicillin	MD	A010
Agarose	Solarbio	A8201
Trypton	Solarbio	T8490
Yeast extract	Solarbio	Y8020
Sodium chloride	CHRON chemicals	7647-14-5
Agar	Solarbio	A8190
Trypan blue dye	Sigma	T8154
RPMI1640	GIBCO	C11875500BT
Activin A	R&D	338-AC
CHIR99021	TOCRIS	4423
BSA	Solarbio	A8010
ITS supplement	Gibco	41400045
Opti-MEM	Gibco	31985070
75% ethanol	Ante	2212134119
Absolute ethanol	Sigma	E7148
Chloroform	Sigma	34854
Isopropanol	Sigma	278475
Nuclease-free water	TIANGEN	RT121
IPTG	Thermo Fisher	15529019
X-gal	Thermo Fisher	R0941
Critical commercial assays
La Taq	TAKARA	Cat# RR52A
Trizol	Gene BioTech	TR118
TIANamp Genomic DNA Kit	TIANGEN	Cat# DP403
PrimeScript RT Master Mix	TAKARA	Cat# RR036A
ChamQ Universal SYBR qPCR Master Mix	Vazyme	Cat# Q311
96-well white plate	Bio-Rad	HSP9601
Lipofectamine™ RNAiMAX kit	Invitrogen	Cat# 13778150
EpiTect Fast Bisulfite Conversion Kits	QIAGEN	Cat# 59824
HiPure Gel DNA Mini Kit	MAGEN	Cat# D211102
pMD™18-T Vector Cloning Kit	TAKARA	Cat# 6011
Experimental models: Cell lines
Human: H1 hESC line	Provided by Jams A. Tomson, University of Wisconsin	WA01
Oligonucleotides
NC-siRNA sense UUCUCCGAACGUGUCACGUdTdT	This paper	N/A
NC-siRNA antisense ACGUGACACGUUCGGAGAAdTdT	This paper	N/A
TET1-siRNA-1 sense GGAGUAAAGCACUCAGAAAdTdT	This paper	N/A
TET1-siRNA-1 antisense UUUCUGAGUGCUUUACUCCdTdT	This paper	N/A
TET1-siRNA-2 sense CGAAGCUACUGCAAAUCAAdTdT	This paper	N/A
TET1-siRNA-2 antisense UUGAUUUGCAGUAGCUUCGdTdT	This paper	N/A
TET1-siRNA-3 sense GAAGAGACAUUGAAUGAUAdTdT	This paper	N/A
TET1-siRNA-3 aitisense UAUCAUUCAAUGUCUCUUCdTdT	This paper	N/A
qPCR Primer TET1-qP-Forward ATCCTTGCTGTGTAGAGTTC	This paper	N/A
qPCR Primer TET1-qP-Reverse AGTATTGGTGATATGCTTCCTAA	This paper	N/A
qPCR Primer LTR6B-qP-Forward CCGAGAGAATTTTGAGCGTTAGC	This paper	N/A
qPCR Primer LTR6B-qP-Reverse TGTTGTACCTGAGCGAGTTAGAG	This paper	N/A
qPCR Primer FOXA2-qP-Forward ACAGCAGTCTTCTTCACC	This paper	N/A
qPCR Primer FOXA2-qP-Reverse AGCAGGAGTCTACACAGTA	This paper	N/A
qPCR Primer SOX17-qP-Forward ACCGCACGGAATTTGAAC	This paper	N/A
qPCR Primer SOX17-qP-Reverse GCAGTAATATACCGCGGAGC	This paper	N/A
qPCR Primer ACTIN-qP-Forward CCCAGAGCAAGAGAGG	This paper	N/A
qPCR Primer ACTIN-qP-Reverse GTCCAGACGCAGGATG	This paper	N/A
BSP primer LTR6B-BS-Forward: AAGAATGTAATTAATTGATAAG	This paper	N/A
BSP primer LTR6B-BS-Reverse: CCCCRAAAAAAAAACTAAATT	This paper	N/A
Primer M13F: GTAAAACGACGGCCAGT	This paper	N/A
Primer M13R: CAGGAAACAGCTATGAC	This paper	N/A
Recombinant DNA
pMD18-T vector	TAKARA	6011
Software and algorithms
QUMA	Kumaki et al.4	http://quma.cdb.riken.jp/
Graph Pad prism	GraphPad	https://www.graphpad.com/scientific- software/prism/
SnapGene	Insightful Science	https://www.snapgene.com