Table 3.
Plasmids used in this study
| Strain | Description | Reference |
|---|---|---|
| pTLL108.1 | PacI::Pro-promoter::sgRNA | (van Leeuwe et al. 2019) |
| pTLL109.2 | sgRNA::Terminator::PacI | (van Leeuwe et al. 2019) |
| pFC332 | Contains cas9, a hygromycin resistance cassette and a PacI site for introducing a sgRNA | (Nødvig et al. 2018; Seekles et al. 2021) |
| pCT001 | agsC cut, 254 bp upstream of agsC CDS start codon | This study |
| pCT002 | agsC cut, 252 bp downstream of agsC CDS stop codon | This study |
| pCT003 | agsE cut, 232 bp upstream of agsE CDS start codon | This study |
| pCT004 | agsE cut, 251 bp downstream of agsE CDS stop codon | This study |
| pJL006 | agsE cut, 712 bp upstream of agsE CDS start codon | This study |
| pJL015 | Tet-on promoter between 1000 bp flanks, the left flank 712–1712 bp upstream of the agsE start codon, while the right flank starts from the start codon | This study |