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. 2023 May 19;40(8):1961–1971. doi: 10.1007/s10815-023-02822-y

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© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 3 — Effects of oocyte removal with or without replacement of OSFs on FSHR, AMH, AREG, EREG, ADAM17, EGFR, PTGS2, TNFAIP6, PTX3, and HAS2 mRNA abundance in cumulus cells from COCs subjected to AREG-stimulated IVM for 4 and 22 h. COCs were cultured intact (COC), oocytectomized (OOX), or oocytectomized with denuded oocytes (OOX + DO). Data represent mRNA abundance of a given target gene relative to the reference genes (RPL15 and CYCA). Bars with different letters are significantly different (p<0.05). Data derive from four independent replicates