FIG. 3.

Western blotting analysis of monoclonal antibodies to ECV N protein. Purified N protein was separated on a 10% polyacrylamide gel and electroblotted onto a nitrocellulose membrane. The strips were incubated with different anti-N protein monoclonal antibodies. Lanes 1 and 2, anti-histidine antibody and mouse anti-ECV polyclonal serum, respectively; lanes 3 to 7, different monoclonal antibody clones (clones 3C9, 7A4, 3C10, 8F2, and 9B8, respectively). The arrow indicates a 50-kDa N-protein band. The higher bands may be aggregates of ECV nucleoprotein expressed in E. coli.